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- Title
Imaging of single light-responsive clock cells reveals fluctuating free-running periods.
- Authors
Carr, Amanda-Jayne F.; Whitmore, David
- Abstract
Zebrafish tissues and cell lines contain circadian clocks that respond directly to light. Using fluorescence-activated cell sorting, we have isolated clonal cell lines that contain the reporter construct, zfperiod4-luciferase. Bioluminescent assays show that oscillations within cell populations are dampened in constant darkness. However, single-cell imaging reveals that individual cells continue to oscillate, but with widely distributed phases and marked stochastic fluctuations in free-running period. Because these cells are directly light responsive, we can easily follow phase shifts to single light pulses. Here we show that light acts to reset desynchronous cellular oscillations to a common phase, as well as stabilize the subsequent free-running period.
- Subjects
PRESERVATION of organs, tissues, etc.; ORGANS (Anatomy); TISSUES; CELLS; CELL culture; CELL lines; OSCILLATIONS
- Publication
Nature Cell Biology, 2005, Vol 7, Issue 3, p319
- ISSN
1465-7392
- Publication type
Article
- DOI
10.1038/ncb1232