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- Title
Increased sarcolemmal Na /H exchange activity in hypertrophied myocytes from dogs with chronic atrioventricular block.
- Authors
van Borren, Marcel M. G. J.; Vos, Marc A.; Houtman, Marien J. C.; Antoons, Gudrun; Ravesloot, Jan H.
- Abstract
Dogs with compensated biventricular hypertrophy due to chronic atrioventricular block (cAVB), are more susceptible to develop drug-induced Torsade-de-Pointes arrhythmias and sudden cardiac death. It has been suggested that the increased Na+ influx in hypertrophied cAVB ventricular myocytes contribute to these lethal arrhythmias. The increased Na+ influx was not mediated by Na+ channels, in fact the Na+ current proved reduced in cAVB myocytes. Here we tested the hypothesis that increased activity of the Na+/H+ exchanger type 1 (NHE-1), commonly observed in hypertrophic hearts, causes the elevated Na+ influx. Cardiac acid-base transport was studied with a pH-sensitive fluorescent dye in ventricular myocytes isolated from control and hypertrophied cAVB hearts; the H+ equivalent flux through NHE-1, Na+-HCO-3 cotransport (NBC), Cl-/OH- exchange (CHE), and Cl-/HCO-3 exchange (AE) were determined and normalized per liter cell water and corrected for surface-to-volume ratio. In cAVB, sarcolemmal NHE-1 flux was increased by 65 ± 6.3% in the pHi interval 6.3-7.2 and NBC, AE, and CHE fluxes remained unchanged. Accordingly, at steady-state intracellular pH the total sarcolemmal Na+ influx by NHE-1 + NBC increased from 8.5 ± 1.5 amol/μm2/min in normal myocytes to 15 ± 2.4 amol/μm2/min in hypertrophied cAVB myocytes. We conclude that compensated cardiac hypertrophy in cAVB dogs is accompanied with an increased sarcolemmal NHE-1 activity. This in conjunction with unchanged activity of the other acid-base transporters will raise the intracellular Na+ in hypertrophied cAVB myocytes.
- Subjects
SODIUM-hydrogen antiporter; SODIUM in the body; ACID-base equilibrium; MUSCLE cells; HYDROGEN-ion concentration; CARDIAC hypertrophy
- Publication
Frontiers in Physiology, 2013, Vol 4, p1
- ISSN
1664-042X
- Publication type
Article
- DOI
10.3389/fphys.2013.00322