We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Half a century of bacteriophage lambda recombinase: In vitro studies of lambda exonuclease and Red‐beta annealase.
- Authors
Brewster, Jodi L.; Tolun, Gökhan
- Abstract
DNA recombination, replication, and repair are intrinsically interconnected processes. From viruses to humans, they are ubiquitous and essential to all life on Earth. Single‐strand annealing homologous DNA recombination is a major mechanism for the repair of double‐stranded DNA breaks. An exonuclease and an annealase work in tandem, forming a complex known as a two‐component recombinase. Redβ annealase and λ‐exonuclease from phage lambda form the archetypal two‐component recombinase complex. In this short review article, we highlight some of the in vitro studies that have led to our current understanding of the lambda recombinase system. We synthesize insights from more than half a century of research, summarizing the state of our current understanding. From this foundation, we identify the gaps in our knowledge and cast an eye forward to consider what the next 50 years of research may uncover.
- Subjects
BACTERIOPHAGE lambda; SINGLE-stranded DNA; RECOMBINASES; RECOMBINANT DNA; DOUBLE-strand DNA breaks; DNA repair; EXONUCLEASES
- Publication
IUBMB Life, 2020, Vol 72, Issue 8, p1622
- ISSN
1521-6543
- Publication type
Article
- DOI
10.1002/iub.2343