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- Title
A novel pectin methylesterase inhibitor, PMEI3, in common bean suggests a key role of pectin methylesterification in Pseudomonas resistance.
- Authors
Rubia, Alfonso G De la; Largo-Gosens, Asier; Yusta, Ricardo; Sepúlveda-Orellana, Pablo; Riveros, Aníbal; Centeno, María Luz; Sanhueza, Dayan; Meneses, Claudio; Saez-Aguayo, Susana; García-Angulo, Penélope
- Abstract
The mechanisms underlying susceptibility to and defense against Pseudomonas syringae (Pph) of the common bean (Phaseolus vulgaris) have not yet been clarified. To investigate these, 15-day-old plants of the variety Riñón were infected with Pph and the transcriptomic changes at 2 h and 9 h post-infection were analysed. RNA-seq analysis showed an up-regulation of genes involved in defense/signaling at 2 h, most of them being down-regulated at 9 h, suggesting that Pph inhibits the transcriptomic reprogramming of the plant. This trend was also observed in the modulation of 101 cell wall-related genes. Cell wall composition changes at early stages of Pph infection were associated with homogalacturonan methylation and the formation of egg boxes. Among the cell wall genes modulated, a pectin methylesterase inhibitor 3 (PvPMEI3) gene, closely related to AtPMEI3 , was detected. PvPMEI3 protein was located in the apoplast and its pectin methylesterase inhibitory activity was demonstrated. PvPMEI3 seems to be a good candidate to play a key role in Pph infection, which was supported by analysis of an Arabidopsis pmei3 mutant, which showed susceptibility to Pph, in contrast to resistant Arabidopsis Col-0 plants. These results indicate a key role of the degree of pectin methylesterification in host resistance to Pph during the first steps of the attack.
- Subjects
PECTINESTERASE; PECTINS; CULTIVARS; PSEUDOMONAS syringae; PSEUDOMONAS; DISEASE resistance of plants; COMMON bean
- Publication
Journal of Experimental Botany, 2024, Vol 75, Issue 1, p364
- ISSN
0022-0957
- Publication type
Article
- DOI
10.1093/jxb/erad362