We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
DNA Barcodes of Mansonia (Mansonia) Blanchard, 1901 (Diptera, Culicidae).
- Authors
Amorim, Jandui Almeida; de Oliveira, Tatiane Marques Porangaba; de Sá, Ivy Luizi Rodrigues; da Silva, Taires Peniche; Sallum, Maria Anice Mureb
- Abstract
Females of the genus Mansonia feed on the blood of humans, livestock, and other vertebrates to develop their eggs. The females' biting behavior may cause severe disturbance to blood hosts, with a negative impact on public health and economics. Certain species have been identified as potential or effective disease vectors. The accurate species identification of field-collected specimens is of paramount importance for the success of monitoring and control strategies. Mansonia (Mansonia) morphological species boundaries are blurred by patterns of intraspecific heteromorphism and interspecific isomorphism. DNA barcodes can help to solve taxonomic controversies, especially if combined with other molecular tools. We used cytochrome c oxidase subunit I (COI) gene 5′ end (DNA barcode) sequences to identify 327 field-collected specimens of Mansonia (Mansonia) spp. The sampling encompassed males and females collected from three Brazilian regions and previously assigned to species based on their morphological characteristics. Eleven GenBank and BOLD sequences were added to the DNA barcode analyses. Initial morphospecies assignments were mostly corroborated by the results of five clustering methods based on Kimura two-parameter distance and maximum likelihood phylogeny. Five to eight molecular operational taxonomic units may represent taxonomically unknown species. The first DNA barcode records for Mansonia fonsecai, Mansonia iguassuensis, and Mansonia pseudotitillans are presented.
- Subjects
AMAZON River Region; MOSQUITOES; GENETIC barcoding; DIPTERA; DNA analysis; CYTOCHROME oxidase; DISEASE vectors
- Publication
Genes, 2023, Vol 14, Issue 6, p1127
- ISSN
2073-4425
- Publication type
Article
- DOI
10.3390/genes14061127