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- Title
Preliminary assessment on Agrobacterium-mediated transformation of Dendrobium Broga Giant orchid's PLBs.
- Authors
Uddain, Jasim; Zakaria, Latiffah; Lynn, Chew Bee; Subramaniam, Sreeramanan
- Abstract
Dendrobium Broga Giant (Dendrobium Bobby Messina × Dendrobium superbiens) is a newly introducing hybrid orchid species in Malaysia. Suitable target explants potentiality determination is very important before transformation studies. Bacterial chemotaxis and transient gusA expression were studied using explants as PLBs of Dendrobium Broga Giant to evaluate the capability of bacterial-PLBs interaction during transformation period. Agrobacterium tumefaciens showed positive chemotactic response and their attachment to the PLBs whereas E. coli responded negatively. Mild wounded PLBs displayed higher Agobacterium motility compared to other treatments. Mild wounded PLBs immersed for 15 minutes on the Agrobacterium suspension (OD600 nm 0.8) and co-cultivated for 2 days in co-cultivation media supplemented with 200μM acetosyringone showed highest transient gusA gene expression in Dendrobium Broga Giant orchid. Hence, we concluded that positive chemotactic responses and transient gusA gene expression ability indicate the Dendrobium Broga Giant (DBG) orchid PLBs are a potential target explants for Agrobacterium-mediated genetic transformation studies.
- Subjects
MALAYSIA; DENDROBIUM; AGROBACTERIUM; ACETOSYRINGONE; GENETIC translation; EDUCATION
- Publication
Emirates Journal of Food & Agriculture (EJFA), 2015, Vol 27, Issue 9, p669
- ISSN
2079-052X
- Publication type
Article
- DOI
10.9755/ejfa.2015.05.211