We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Determination of the activity of superoxide dismutase using a glassy carbon electrode modified with ferrocene imidazolium salts and hydroxy-functionalized graphene.
- Authors
Wang, Jian; Jiang, Zhou; Xie, Lili; Liu, Minyan; Yuan, Yaofeng
- Abstract
A glassy carbon electrode was modified with a composite consisting of ferrocene imidazolium salts and hydroxy-functionalized graphene in a Nafion matrix. The electrode is shown to facilitate the reduction of oxygen to form the superoxide anion radical. Atomic force microscopy and electrochemical impedance spectroscopy were used to characterize the electrode. The in-situ production of superoxide anion was then studied with an analogue of methyl-Cypridina luciferin (also referred to as Vargulin). As a result, an electrochemiluminescence (ECL) based assay was developed for the improved determination of the activity of the enzyme superoxide dismutase (SOD). Under optimized conditions, the ECL based calibration plot is linear in the 0.5 to 6.5 U·mL SOD activity range, with a 0.2 U·mL detection limit (at a signal-to-noise ratio of 3).
- Subjects
SUPEROXIDE dismutase; CARBON electrodes; FERROCENE; GRAPHENE; NAFION; ELECTROCHEMILUMINESCENCE; ATOMIC force microscopy; SIGNAL-to-noise ratio
- Publication
Microchimica Acta, 2017, Vol 184, Issue 1, p289
- ISSN
0026-3672
- Publication type
Article
- DOI
10.1007/s00604-016-2018-x