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- Title
Altered anionic GBM components in monoclonal antibody against slit diaphragm-injected proteinuric rats.
- Authors
Fujigaki, Yoshihide; Nagase, Mitsumasa; Hidaka, Sumi; Matsui, Katsuyuki; Shirai, Mikiko; Nosaka, Hitonari; Kawachi, Hiroshi; Shimizu, Fujio; Hishida, Akira
- Abstract
Altered anionic GBM components in monoclonal antibody against slit diaphragm injected proteinuric rats. Background . We previously reported that monoclonal antibody (mAb) 5-1-6 bound to renal filtration slits induces massive proteinuria without causing ultrastructural changes in the glomerulus. This study evaluated the underlying mechanisms of the increase in glomerular permeability. Methods . The distribution of endogenous albumin and IgG in the glomerular basement membrane (GBM) was studied in in situ drip-fixed glomeruli of Munich-Wistar rats by use of immunogold immunocytochemistry in the presence and absence of mAb 5-1-6. The density of foot process glycocalyx components was estimated by labeling with Limax fluvus lectin- or Helix pomatia lectin-gold complexes. Anionic sites in the GBM were examined by labeling with cationic gold at pH 2.0 or 7.4. Carboxyl groups, which also furnish an anionic charge to the GBM, were examined by specific biotinylation and colloidal gold probe methods. In addition, the infusion-staining of anionic sites was performed by use of ruthenium red in both Munich-Wistar and Wistar rats. Results . The urinary excretion of albumin and IgG was increased markedly in the treated rats, indicating a non-selective barrier defect. In the control rats, albumin and IgG molecules were mainly located along the inner half of the GBM, and to a lesser degree in the lamina rara externa. In the treated rats, the albumin and IgG moieties were more equally distributed throughout the width of the GBM. Newly appearing, small dense peaks at the outer side of the GBM were evident, indicating a barrier function of outer zone of the GBM and/or epithelial cell layer. No intergroup differences in the density of lectin binding sites on foot processes were seen. The reduction in the number of ruthenium red-positive anionic sites and cationic gold (pH 2.0)-labeled anionic sites in the lamina rara externa was significant in the treated rats at day 3, indicating a...
- Subjects
BASAL lamina; MONOCLONAL antibodies
- Publication
Kidney International, 1998, Vol 54, Issue 5, p1491
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1046/j.1523-1755.1998.00131.x