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- Title
A.actinomycetemcomitans-induced periodontal disease promotes systemic and local responses in rat periodontium.
- Authors
Bezerra, Beatriz de Brito; Andriankaja, Oelisoa; Kang, Jun; Pacios, Sandra; Bae, Hyung Jin; Li, Yu; Tsiagbe, Vincent; Schreiner, Helen; Fine, Daniel H.; Graves, Dana T.
- Abstract
Aim To characterize the histologic and cellular response to A. actinomycetemcomitans ( Aa) infection. Material & Methods Wistar rats infected with Aa were evaluated for antibody response , oral Aa colonization, loss of attachment, PMN recruitment, TNF-α in the junctional epithelium and connective tissue, osteoclasts and adaptive immune response in local lymph nodes at baseline and 4, 5 or 6 weeks after infection. Some groups were given antibacterial treatment at 4 weeks. Results An antibody response against Aa occurred within 4 weeks of infection, and 78% of inoculated rats had detectable Aa in the oral cavity ( p < 0.05). Aa infection significantly increased loss of attachment that was reversed by antibacterial treatment ( p < 0.05). TNF-α expression in the junctional epithelium followed the same pattern. Aa stimulated high osteoclast formation and TNF-α expression in the connective tissue ( p < 0.05). PMN recruitment significantly increased after Aa infection ( p < 0.05). Aa also increased the number of CD8+ T cells ( p < 0.05), but not CD4+ T cells or regulatory T cells (Tregs) ( p > 0.05). Conclusion Aa infection stimulated a local response that increased numbers of PMNs and TNF-α expression in the junctional epithelium and loss of attachment. Both TNF-α expression in JE and loss of attachment was reversed by antibiotic treatment. Aa infection also increased TNF-α in the connective tissue, osteoclast numbers and CD8+ T cells in lymph nodes. The results link Aa infection with important characteristics of periodontal destruction.
- Subjects
RISK factors of periodontal disease; INFLAMMATION; ACTINOMYCETALES; ANALYSIS of variance; ANIMAL experimentation; ANTIBIOTICS; BACTERIAL diseases; BIOLOGICAL models; CHI-squared test; FISHER exact test; IMMUNOHISTOCHEMISTRY; RATS; RESEARCH funding; T cells; T-test (Statistics); U-statistics
- Publication
Journal of Clinical Periodontology, 2012, Vol 39, Issue 4, p333
- ISSN
0303-6979
- Publication type
Article
- DOI
10.1111/j.1600-051X.2011.01847.x