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- Title
더덕 및 씀바귀 참조전사체 단일 파일 DB 구축.
- Authors
이태호; 오윤호; 강지남; 이시명
- Abstract
In this study, we constructed a reference transcriptome database for the special crops Codonopsis lanceolata (known as “deodeok” in Korean) and Ixeridium dentata (known as “sseumbagwi” in Korean). The reference transcriptome sequence based on PacBio Iso-Seq technology was rapidly analyzed using various programs on a supercomputer at the Rural Development Administration to determine homologous gene searches, domain searches, and metabolic pathway-related information. Transcriptome information was organized into a single file database (DB) with a simple user interface using Microsoft Access, enabling efficient access and management of transcriptome sequences and functional prediction information. The deodeok reference transcriptome DB included 49,677 transcriptome sequences, whereas the sseumbagwi reference transcriptome DB included 73,757 transcriptome sequences. These reference transcriptome DBs can be utilized for various genomic analyses, such as gene discovery, gene expression analysis, inter-and intraspecies comparisons, and marker development, with particular potential for use in secondary metabolite research. The reference transcriptome DBs constructed in this study are expected to contribute to enhancing the agricultural value and industrial applicability of deodeok and sseumbagwi.
- Subjects
SPECIALTY crops; GENOMICS; SECONDARY research; GENE expression; AGRICULTURE; SUPERCOMPUTERS
- Publication
Korean Journal of Breeding Science, 2023, Vol 55, Issue 4, p321
- ISSN
0250-3360
- Publication type
Article
- DOI
10.9787/KJBS.2023.55.4.321