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- Title
Genetic Diversity Estimation of the Rice Mutant Lines Induced by Sodium Azide.
- Authors
Young-Seop Shin; Ji-Ung Jeung
- Abstract
To investigate dose-effect of a chemical mutagen, sodium azide on a rice elite line, Suweon472, seed aliquots were treated with five different concentrations of sodium azide. The degree of mutation levels of each aizde concentration were estimated by using DNA fingerprinting techniques such as RAPD and AFLP. Some selected mutant lines (M4) were also subjected for DNA fingerprinting to estimate their mutation levels by comparing the banding patterns of the wild type, Suweon 472. RAPD and AFLP fingerprinting patterns indicated that dose-effect of different azide concentrations was not clear. With allele description of detected AFLPs among favorable mutant lines, it was possible to discriminate each mutant line from others which have similar phenotypes and reactions against pathogens. AFLP fingerprinting patterns of waxy mutant lines, otherwise, were highly homogeneous as well as their phenotypic and agronomic characters.
- Subjects
PLANT diversity; PHYSIOLOGICAL effects of sodium; AZIDES; RICE varieties; PLANT mutation; AMPLIFIED fragment length polymorphism
- Publication
Korean Journal of Breeding Science, 2011, Vol 43, Issue 1, p23
- ISSN
0250-3360
- Publication type
Article