We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Morphological and molecular identification of plant pathogenic fungi associated with necrotic wood tissues of pomegranate trees in Iran.
- Authors
Kadkhoda-Hematabadi, Somayeh; Mohammadi, Hamid; Sohrabi, Mahboobeh
- Abstract
Pomegranate is an economically important horticultural crop tree in tropical and subtropical countries. In 2016 and 2017, a survey of pomegranate fungal trunk pathogens was conducted in 20 orchards in Kerman and Fars provinces in Iran. Branch samples were collected from affected trees exhibiting yellowing, canker, sparse foliage, dieback, and decline. Fungal isolations were made from the margin between healthy and necrotic wood tissues on potato dextrose agar (PDA). All fungal isolates were identified based on morphological and cultural characteristics. Morphological identifications of the representative isolates were confirmed by sequence analysis of the internal transcribed spacer (ITS) nrDNA, partial β-tubulin gene (tub2), actin (act) and a partial sequence of the translation elongation factor 1α (tef-1α). In total, 285 fungal isolates were obtained from infected tissues, and the species Phaeoacremonium (P.) parasiticum, P. alvesii, P. rubrigenum, P. italicum, P. fraxinopennsylvanicum, P. inflatipes, Neoscytalidium (N.) dimidiatum, Lasiodiplodia (L.) theobromae, Diatrype (D.) whitmanensis, Kalmusia variispora, Neomicrosphaeropsis italica and Stilbocrea walteri were identified. Pathogenicity tests were conducted on pomegranate shoots, and the length, width and depth of the wood lesions caused by inoculated isolates were assessed 40 days after inoculation. The lesions caused by L. theobromae were significantly longer (F = 4.29, P < 0.0012) than those caused by other species while, N. dimidiatum caused wider (F = 6.27, P < 0.0001) and deeper (F = 5.18, P < 0.0003) wood lesions compared to other tested species. This study represents the first record of P. alvesii, P. rubrigenum, P. fraxinopennsylvanicum, P. inflatipes, D. whitmanensis, K. variispora, N. italica and S. walteri on pomegranate trees worldwide and the first report of N. italica in Iran.
- Subjects
IRAN; WOOD; PHYTOPATHOGENIC fungi; PLANT identification; PATHOGENIC fungi; PLANT-fungus relationships; LEAF spots; POMEGRANATE
- Publication
Journal of Plant Pathology, 2023, Vol 105, Issue 2, p465
- ISSN
1125-4653
- Publication type
Article
- DOI
10.1007/s42161-023-01308-1