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- Title
Changes in Glucose Metabolism in Vertical Sleeve Gastrectomy.
- Authors
Lho, Yunmee; Roux, Carel; Park, Hyeon; Kim, Gon; Jung, Jeeyoun; Hwang, Geum-Sook; Seo, Youn; Ha, Tae; Ha, Eunyoung
- Abstract
Background: We evaluated metabolic changes after vertical sleeve gastrectomy (VSG) surgery in a rat model using proteomics and metabolomic profiling in liver and serum. Methods: Rats were randomly divided into two groups: sham ( n = 10) and VSG ( n = 12). Food intake, body weight, blood glucose, insulin, and thyroid hormone levels were measured. Two-dimensional electrophoresis, nuclear resonance spectroscopy, mass spectroscopy, immunofluorescence, and immunoblot analyses were used to determine and validate changes in metabolites and proteins in liver tissue and serum samples. Results: Food intake and body weight decreased after VSG group ( p < 0.05 and p < 0.05, respectively). Random blood glucose (sham, 183.3 ± 5.6 mg/dL; VSG, 138.5 ± 3.7 mg/dL) decreased while random insulin (sham, 0.45 ± 0.16 μg/L; VSG, 1.05 ± 0.18 μg/L) increased after VSG ( p < 0.05 and p < 0.01, respectively). We found that expressions of gluconeogenic enzymes (phosphoenolpyruvate carboxykinase-1 and glucose-6-phosphatase) and concentrations of pyruvate and malate decreased while lactate, NADH, NADPH, glucose, and AMP/ATP ratio increased after VSG. Thyroid hormones, triiodothyronine (T3) and free thyroxine (fT4), decreased after VSG. Conclusion: This study proves that VSG suppresses hepatic glucose production.
- Subjects
GLUCOSE metabolism; GASTRECTOMY; PROTEOMICS; METABOLOMICS; SERUM
- Publication
Obesity Surgery, 2015, Vol 25, Issue 11, p2002
- ISSN
0960-8923
- Publication type
Article
- DOI
10.1007/s11695-015-1636-4