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- Title
Metabolic engineering of the 2-ketobutyrate biosynthetic pathway for 1-propanol production in <italic>Saccharomyces cerevisiae</italic>.
- Authors
Nishimura, Yuya; Matsui, Terumi; Ishii, Jun; Kondo, Akihiko
- Abstract
Background: To produce 1-propanol as a potential biofuel, metabolic engineering of microorganisms, such as <italic>E. coli</italic>, has been studied. However, 1-propanol production using metabolically engineered <italic>Saccharomyces cerevisiae</italic>, which has an amazing ability to produce ethanol and is thus alcohol-tolerant, has infrequently been reported. Therefore, in this study, we aimed to engineer <italic>S. cerevisiae</italic> strains capable of producing 1-propanol at high levels. Results: We found that the activity of endogenous 2-keto acid decarboxylase and alcohol/aldehyde dehydrogenase is sufficient to convert 2-ketobutyrate (2 KB) to 500 mg/L 1-propanol in yeast. Production of 1-propanol could be increased by: (i) the construction of an artificial 2 KB biosynthetic pathway from pyruvate via citramalate (<italic>cimA</italic>); (ii) overexpression of threonine dehydratase (<italic>tdcB</italic>); (iii) enhancement of threonine biosynthesis from aspartate (<italic>thrA</italic>, <italic>thrB</italic> and <italic>thrC</italic>); and (iv) deletion of the <italic>GLY1</italic> gene that regulates a competing pathway converting threonine to glycine. With high-density anaerobic fermentation of the engineered <italic>S. cerevisiae</italic> strain YG5C4231, we succeeded in producing 180 mg/L 1-propanol from glucose. Conclusion: These results indicate that the engineering of a citramalate-mediated pathway as a production method for 1-propanol in <italic>S. cerevisiae</italic> is effective. Although optimization of the carbon flux in <italic>S. cerevisiae</italic> is necessary to harness this pathway, it is a promising candidate for the large-scale production of 1-propanol.
- Subjects
PROPANOLS; BIOSYNTHESIS; SACCHAROMYCES cerevisiae; BIOMASS energy; ALTERNATIVE fuels
- Publication
Microbial Cell Factories, 2018, Vol 17, p1
- ISSN
1475-2859
- Publication type
Article
- DOI
10.1186/s12934-018-0883-1