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- Title
The BICD2 dynein cargo adaptor binds to the HPV16 L2 capsid protein and promotes HPV infection.
- Authors
Speckhart, Kaitlyn; Choi, Jeongjoon; DiMaio, Daniel; Tsai, Billy
- Abstract
During entry, human papillomavirus (HPV) traffics from the endosome to the trans Golgi network (TGN) and Golgi and then the nucleus to cause infection. Although dynein is thought to play a role in HPV infection, how this host motor recruits the virus to support infection and which entry step(s) requires dynein are unclear. Here we show that the dynein cargo adaptor BICD2 binds to the HPV L2 capsid protein during entry, recruiting HPV to dynein for transport of the virus along the endosome-TGN/Golgi axis to promote infection. In the absence of BICD2 function, HPV accumulates in the endosome and TGN and infection is inhibited. Cell-based and in vitro binding studies identified a short segment near the C-terminus of L2 that can directly interact with BICD2. Our results reveal the molecular basis by which the dynein motor captures HPV to promote infection and identify this virus as a novel cargo of the BICD2 dynein adaptor. Author summary: HPV traffics from the endosome, the TGN/Golgi, and then the nucleus to cause infection, although how this is accomplished is unclear. In this manuscript, we demonstrate that the dynein cargo adaptor BICD2 interacts directly with the HPV L2 capsid protein, ferrying the virus from the endosome to the TGN/Golgi in order to promote infection.
- Subjects
HUMAN papillomavirus; DYNEIN; ADAPTOR proteins; MOLECULAR motor proteins; FREIGHT &; freightage; VIRUS diseases; PROTEINS
- Publication
PLoS Pathogens, 2024, Vol 20, Issue 6, p1
- ISSN
1553-7366
- Publication type
Article
- DOI
10.1371/journal.ppat.1012289