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- Title
Novel miR390-Dependent Transacting siRNA Precursors in Plants Revealed by a PCR-Based Experimental Approach and Database Analysis.
- Authors
Krasnikova, M. S.; Milyutina, I. A.; Bobrova, V. K.; Ozerova, L. V.; Troitsky, A. V.; Solovyev, A. G.; Morozov, S. Y.
- Abstract
TAS loci in plant genomes encode transacting small interfering RNAs (ta-siRNAs) that regulate expression of a number of genes. The function of TAS3 precursor in Arabidopsis thaliana is controlled by two miR390 target sites flanking two ta-siARF sequences targeting mRNAs of ARF transcription factors. Cleavage of the 3′-miR390-site initiates ta-siRNAs biogenesis. Here we describe the new method for identification of plant ta-siRNA precursors based on PCR with oligodeoxyribonucleotide primers mimicking miR390. The method was found to be efficient for dicotiledonous plants, cycads, and mosses. Based on sequences of amplified loci and a database analysis, a novel type of miR390-dependent TAS sequences was identified in dicots. These TAS loci are characterized by a smaller distance between miR390 sites compared to TAS3, a single copy of ta-siARF, and a sequence conservation pattern pointing to the possibility that processing of novel TAS-like locus is initiated by cleavage of the 5′-terminal miR390 target site.
- Subjects
GENE expression in plants; SMALL interfering RNA; GENETIC regulation in plants; POLYMERASE chain reaction; DICOTYLEDONS; NUCLEOTIDE sequence; SCISSION (Chemistry)
- Publication
Journal of Biomedicine & Biotechnology, 2009, p1
- ISSN
1110-7243
- Publication type
Article
- DOI
10.1155/2009/952304