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- Title
Expression and localization of silkworm adaptor protein complex-1 subunits, which were down-regulated post baculovirus infection.
- Authors
Niu, Yan-shan; Wang, Mei-xian; Liang, Shuang; Zhou, Fang; Miao, Yun-gen
- Abstract
Adaptor protein complexes (APs) function as vesicle coat components in different membrane traffic pathways. In this study the subunits of adaptor protein complex-1 (AP-1) of silkworm Bombyx mori were molecularly characterized. All coding genes for the four subunits were cloned and sequenced. Phylogenic tree for each adaptin was constructed and all subunits were found to be conserved in respective group among organisms. The mRNA expression pattern for each adaptin was similar among tissues. Alternative splicing event was observed in genes encoding both the heavy chain gamma and beta adaptin and the light chain subunit, which could generate other possible adaptin forms. GFP-tagged fusion proteins indicated that AP-1 located in the peripheral plasma area. Furthermore, the BmNPV infection in B. mori cells had differentiated effect on the expression level of AP-1 subunits.
- Subjects
ADAPTOR proteins; BACULOVIRUS genetics; MESSENGER RNA; SILKWORMS; PHYLOGENY
- Publication
Molecular Biology Reports, 2012, Vol 39, Issue 12, p10775
- ISSN
0301-4851
- Publication type
Article
- DOI
10.1007/s11033-012-1971-7