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- Title
Loss of DNA methylation affects somatic embryogenesis in Medicago truncatula.
- Authors
Santos, D.; Fevereiro, P.
- Abstract
To investigate the involvement of methylation of DNA in somatic embryogenesis we initiated a comparative study using Medicago truncatula lines that have different capacities to produce somatic embryos. Treatment with the demethylating drug 5-azacytidine caused a loss of regeneration capacity in the embryogenic line by arresting the production of somatic embryos. Analysis with methylation-sensitive enzymes showed disruption of somatic embryogenesis competence to be correlated with rDNA demethylation. Our data suggest production of somatic embryos depends on a certain level of DNA methylation.
- Publication
Plant Cell, Tissue & Organ Culture, 2002, Vol 70, Issue 2, p155
- ISSN
0167-6857
- Publication type
Article
- DOI
10.1023/A:1016369921067