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- Title
Spheroid model study comparing the biocompatibility of Biodentine and MTA.
- Authors
Pérard, Matthieu; Clerc, Justine; Meary, Fleur; Pérez, Fabienne; Tricot-Doleux, Sylvie; Pellen-Mussi, Pascal
- Abstract
The primary objective of this study was to assess the biological effects of a new dentine substitute based on CaSiO (Biodentine™) for use in pulp-capping treatment, on pseudo-odontoblastic (MDPC-23) and pulp (Od-21) cells. The secondary objective was to evaluate the effects of Biodentine and mineral trioxide aggregate (MTA) on gene expression in cultured spheroids. We used the acid phosphatase assay to compare the biocompatibility of Biodentine and MTA. Cell differentiation was investigated by RT-qPCR. We investigated the expression of genes involved in odontogenic differentiation ( Runx2), matrix secretion ( Col1a1, Spp1) and mineralisation ( Alp). ANOVA and PLSD tests were used for data analysis. MDPC-23 cells cultured in the presence of MTA had higher levels of viability than those cultured in the presence of Biodentine and control cells on day 7 ( P = 0.0065 and P = 0.0126, respectively). For Od-21 cells, proliferation rates on day 7 were significantly lower in the presence of Biodentine or MTA than for control ( P < 0.0001). Col1a1 expression levels were slightly lower in cells cultured in the presence of MTA than in those cultured in the presence of Biodentine and in control cells. Biodentine and MTA may modify the proliferation of pulp cell lines. Their effects may fluctuate over time, depending on the cell line considered. The observed similarity between Biodentine and MTA validates the indication for direct pulp-capping claimed by the manufacturers.
- Subjects
BIOCOMPATIBILITY; DENTAL materials; DENTIN; CALCIUM compounds; ODONTOBLASTS; GENE expression; CELL proliferation
- Publication
Journal of Materials Science: Materials in Medicine, 2013, Vol 24, Issue 6, p1527
- ISSN
0957-4530
- Publication type
Article
- DOI
10.1007/s10856-013-4908-3