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- Title
Carbon nanodots combined with loop-mediated isothermal amplification (LAMP) for detection of African swine fever virus (ASFV).
- Authors
Cao, Gaihua; Qiu, Yue; Long, Keyi; Xiong, Yifan; MeimeiShi; JunYang; Li, Yingguo; Nie, Fuping; Huo, Danqun; Hou, Changjun
- Abstract
The spread of African swine fever virus (ASFV) caused huge economic costs, so early detection is particularly important. Here, we established a fluorescence biosensor based on carbon nanodots (CNDs) and loop-mediated isothermal amplification (LAMP) to ultra-sensitively detect ASFV. LAMP with high efficiency produced a large amount of pyro phosphoric acid and caused pH change in a short time. CNDs with strong light stability had a large fluorescence response at the emission wavelength of 585.5 nm to small pH change by the excitation wavelength of 550 nm. The biosensor realized "turn-off–on" mode for ASFV detection with the detection limit as low as 15.21 copies μL−1. In addition, the biosensor had high accuracy in the actual sample assay. Therefore, the biosensor achieved rapid, sensitive, low-cost, and simple detection for ASFV. Moreover, the biosensor broadened the detection pathway of LAMP as a tool with great development prospect.
- Subjects
CARBON nanodots; AFRICAN swine fever virus; ELECTROCHEMILUMINESCENCE; LAMPS
- Publication
Microchimica Acta, 2022, Vol 189, Issue 9, p1
- ISSN
0026-3672
- Publication type
Article
- DOI
10.1007/s00604-022-05390-7