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- Title
Nitrogen Fixation in Extracts of Azotobacter vinelandii.
- Authors
NICHOLAS, D. J. D.; FISHER, D. J.
- Abstract
IT is well established that soil micro-organisms, including species of Azotobacter and Clostridia, fix atmospheric nitrogen. Although several attempts have been made to demonstrate the incorporation of nitrogen-15 into extracts of these micro-organisms the results at best have been variable and of doubtful significance1. Protoplasts, prepared from Azotobacter by careful lysis of the cells with lysozyme, incorporated only small amounts of nitrogen-15, namely, an enrichment of 0.04 atom per cent excess2. We have recently succeeded in preparing cell-free extracts from Azotobacter vinelandii, either by treating the cells with lysozyme or with the ultrasonic probe, which will incorporate nitrogen-15 at a measurable rate. The cells must, however, be disrupted in the culture medium in which they were grown.
- Publication
Nature, 1960, Vol 186, Issue 4726, p735
- ISSN
0028-0836
- Publication type
Article
- DOI
10.1038/186735a0