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- Title
流化床气流超微粉碎协同超高压法提取赶黄草 黄酮工艺优化及其保肝作用研究.
- Authors
孙 艳; 丁昱婵; 秦令祥; 曹 源; 高愿军
- Abstract
Objective To optimize the extraction process of Penthorum chinense flavonoids by fluidized bed jet milling assisted ultrahigh pressure extraction method, and study its liver protecting effect. Methods The fluidized bed jet milling assisted ultrahigh pressure extraction method was used to extract Penthorum chinense flavonoids. And the extraction process parameters were optimized through single factor and response surface tests. At the same time, mice were randomly divided into normal control group, CCl4 model group, biphenyl diester group, and low, medium, and high dose groups of Penthorum chinense flavonoids. Except for the normal control group, the remaining 5 groups were injected with 0.1% CCl4 to construct a liver injury mouse model. The aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH) values and malondialdehyde (MDA) content in mouse serum, as well as the activities of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) in liver tissue were measured by using a fully automated biochemical analyzer. Results The optimum extraction conditions were: Solvent ratio of 25, pressure of 400 MPa, and pressure holding time of 13 minutes. Under these conditions, the yield of Penthorum chinense flavonoids was 3.98%, and the extraction effect of this method was superior to the other 3 kinds of single extraction methods. The results of in vitro liver protection test showed that Penthorum chinense flavonoids could significantly reduce the values of AST, ALT, LDH and MDA content in serum of CCl4 induced liver injury mice, and increased the activities of GSH-Px and SOD in liver tissue of mice. This indicated that Penthorum chinense flavonoids had a good protective effect on CCl4 induced liver injury. Conclusion The optimized extraction conditions can effectively extract Penthorum chinense flavonoids, and the obtained Penthorum chinense flavonoids has a significant liver protective effect.
- Publication
Journal of Food Safety & Quality, 2023, Vol 14, Issue 16, p277
- ISSN
2095-0381
- Publication type
Article