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- Title
Marine Sediment-Derived Streptomyces Strain Produces Angucycline Antibiotics against Multidrug-Resistant Staphylococcus aureus Harboring SCCmec Type 1 Gene.
- Authors
Sabido, Edna M.; Tenebro, Chuckcris P.; Suarez, Angelica Faith L.; Ong, Sarah Diane C.; Trono, Dana Joanne Von L.; Amago, Diana S.; Evangelista, Jose E.; Reynoso, Ann Marielle Q.; Villalobos, Ivy Grace M.; Alit, Luigi Dan D.; Surigao, Cherryl F.; Villanueva, Christelle A.; Saludes, Jonel P.; Dalisay, Doralyn S.
- Abstract
The Philippine archipelago is geographically positioned in the tropics with rich areas of marine biodiversity. Its marine sediments harbor actinomycetes that exhibit antibacterial activity. Screening of actinomycetes isolated from marine sediments collected near the coast of Islas de Gigantes, Iloilo showed one isolate that exhibited high activity against the multidrug-resistant Staphylococcus aureus (MRSA) strain carrying the Staphylococcal Cassette Chromosome mec (SCCmec) type 1 gene, a biomarker for drug resistance. The isolate was identified as Streptomyces sp. strain DSD011 based on its 16s rRNA and protein-coding genes (atpD, recA, rpoB, and trpB) sequences, and was found to be a new species of salt-tolerant marine Streptomyces. Further, the strain harbors both non-ribosomal peptide synthetase (NRPS) and type II polyketide synthase (PKS) in its genome. The targeted chromatographic isolation and chemical investigations by Liquid Chromatography Mass Spectrometry-Time of Flight (LCMS-TOF), tandem mass spectrometry (MS/MS), and Global Natural Product Social molecular networking (GNPS) of the antibiotics produced by the strain afforded the two polycyclic aromatic polyketide angucycline glycosides, fridamycin A (1) and fridamycin D (2), which are products of type II PKS biosynthesis. Compounds 1 and 2 displayed antibacterial activity against MRSA with minimum inhibitory concentration (MIC) of 500 μg/mL and 62.5 μg/mL, respectively. These results suggest that the underexplored marine sediments near the coast of Islas de Gigantes, Iloilo offer access to undiscovered Streptomyces species that are invaluable sources of antibiotic leads.
- Subjects
METHICILLIN-resistant staphylococcus aureus; STREPTOMYCES; ANTIBIOTICS; TANDEM mass spectrometry; MARINE sediments; STAPHYLOCOCCUS aureus
- Publication
Journal of Marine Science & Engineering, 2020, Vol 8, Issue 10, p734
- ISSN
2077-1312
- Publication type
Article
- DOI
10.3390/jmse8100734