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- Title
Preferential gene transcription of T helper 2 cytokines in peripheral CCR4<sup>+</sup>CD4<sup>+</sup> lymphocytes in dogs.
- Authors
Iio, Aki; Motohashi, Tsutomu; Kunisada, Takahiro; Yasuhira, Yuma; Kamishina, Hiroaki; Maeda, Sadatoshi
- Abstract
Background Previous studies reported the involvement of CC chemokine receptor 4 (CCR4)-positive CD4+ cells in the pathogenesis of canine atopic dermatitis. In humans, CCR4 is selectively expressed on type 2 helper T (Th2) cells; however, a subset of canine CCR4+ helper T cells has not been determined. Hypothesis/Objectives To characterize the transcription profile of CCR4+CD4+ lymphocytes isolated from the peripheral blood of healthy dogs. Animals Three healthy dogs were used. Methods The transcription levels of type 1 helper T (Th1) and Th2 cytokines in CCR4+CD4+ and CCR4−CD4+ lymphocytes isolated from healthy dogs were quantified by real-time RT-PCR. Results The CCR4+CD4+ lymphocytes preferentially transcribed Th2 cytokines, such as interleukin-4 and interleukin-13, but not Th1 cytokines, such as interferon-γ. Conclusions and clinical importance CCR4 can be used as a specific marker of Th2 cells for elucidation of the pathogenesis or the establishment of novel therapeutics in canine Th2-associated diseases, such as canine atopic dermatitis.
- Subjects
CYTOKINES; SKIN inflammation; DOG diseases; FLEAS as carriers of disease; ANTIPRURITICS; THERAPEUTICS
- Publication
Veterinary Dermatology, 2014, Vol 25, Issue 3, p199
- ISSN
0959-4493
- Publication type
Article
- DOI
10.1111/vde.12125