We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Nuclear cyclin D1 overexpression is a critical event associated with cell proliferation and invasive growth in gallbladder carcinogenesis.
- Authors
Itoi, Takao; Shinohara, Yasushi; Takeda, Kazuya; Nakamura, Kazuto; Takei, Kazuo; Sanada, Jun; Horibe, Toshiya; Saito, Toshihiko; Kasuya, Kazuhiko; Ebihara, Yoshirou; Itoi, T; Shinohara, Y; Takeda, K; Nakamura, K; Takei, K; Sanada, J; Horibe, T; Saito, T; Kasuya, K; Ebihara, Y
- Abstract
Cyclin D1 overexpression is remarkably frequent in several human carcinomas and is believed to be a critical event in oncogenesis. We examined cyclin D1 expression, p53 expression, and the Ki-67 labeling index by immunostaining in human gallbladder mucosa in conditions varying from normal to malignant tissue. We also examined K-ras codon 12 mutations in these tissues with a two-step polymerase chain reaction. Nuclear cyclin D1 overexpression was observed in 48% of carcinomas occurring independently of adenoma, but not in adenomas, carcinomas arising in adenomas, or nonneoplastic lesions. Cytoplasmic cyclin D1 overexpression was observed in about 15% of abnormal specimens, irrespective of the type of epithelial abnormality. Carcinomas showing nuclear cyclin D1 overexpression had significantly higher Ki-67 labeling indexes than those with no overexpression. Moderately to poorly differentiated adenocarcinomas showed a higher incidence of nuclear cyclin D1 overexpression than papillary to well differentiated carcinomas. Specimens with cyclin D1 overexpression showed a high incidence of lymph permeation, venous permeation, and lymph node metastasis. We conclude that nuclear cyclin D1 overexpression is a critical event importantly associated with cell proliferation and invasive growth in gallbladder carcinogenesis, and that cyclin D1 immunostaining may become a useful marker for evaluating gallbladder carcinomas.
- Subjects
CYCLINS; GALLBLADDER cancer; GASTROENTEROLOGY
- Publication
Journal of Gastroenterology, 2000, Vol 35, Issue 2, p142
- ISSN
0944-1174
- Publication type
journal article
- DOI
10.1007/s005350050027