We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Antimicrobial peptide derived from insulin‐like growth factor‐binding protein 5 improves diabetic wound healing.
- Authors
Yue, Hainan; Song, Pu; Sutthammikorn, Nutda; Umehara, Yoshie; Trujillo‐Paez, Juan Valentin; Nguyen, Hai Le Thanh; Takahashi, Miho; Peng, Ge; Ikutama, Risa; Okumura, Ko; Ogawa, Hideoki; Ikeda, Shigaku; Niyonsaba, François
- Abstract
Impaired keratinocyte functions are major factors that are responsible for delayed diabetic wound healing. In addition to its antimicrobial activity, the antimicrobial peptide derived from insulin‐like growth factor‐binding protein 5 (AMP‐IBP5) activates mast cells and promotes keratinocyte and fibroblast proliferation and migration. However, its effects on diabetic wound healing remain unclear. Human keratinocytes were cultured in normal or high glucose milieus. The production of angiogenic growth factor and cell proliferation and migration were evaluated. Wounds in normal and streptozotocin‐induced diabetic mice were monitored and histologically examined. We found that AMP‐IBP5 rescued the high glucose‐induced attenuation of proliferation and migration as well as the production of angiogenin and vascular endothelial growth factors in keratinocytes. The AMP‐IBP5‐induced activity was mediated by the epidermal growth factor receptor, signal transducer and activator of transcription 1 and 3, and mitogen‐activated protein kinase pathways, as indicated by the inhibitory effects of pathway‐specific inhibitors. In vivo, AMP‐IBP5 markedly accelerated wound healing, increased the expression of angiogenic factors and promoted vessel formation in both normal and diabetic mice. Overall, the finding that AMP‐IBP5 accelerated diabetic wound healing by protecting against glucotoxicity and promoting angiogenesis suggests that AMP‐IBP5 might be a potential therapeutic target for treating chronic diabetic wounds.
- Subjects
WOUND healing; BLOOD; PROTEIN kinases; CONNECTIVE tissue growth factor; CELL culture; IN vivo studies; ANIMAL experimentation; EPIDERMAL growth factor receptors; ANTIMICROBIAL peptides; BLOOD sugar; CELL motility; CELLULAR signal transduction; PATHOLOGIC neovascularization; CELL proliferation; KERATINOCYTES; MICE
- Publication
Wound Repair & Regeneration, 2022, Vol 30, Issue 2, p232
- ISSN
1067-1927
- Publication type
Article
- DOI
10.1111/wrr.12997