We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Proteomic profiling of maize opaque endosperm mutants reveals selective accumulation of lysine-enriched proteins.
- Authors
Morton, Kyla J.; Shangang Jia; Chi Zhang; Holding, David R.
- Abstract
Reduced prolamin (zein) accumulation and defective endoplasmic reticulum (ER) body formation occurs in maize opaque endosperm mutants opaque2 (o2), floury2 (fl2), defective endosperm*B30 (DeB30), and Mucronate (Mc), whereas other opaque mutants such as opaque1 (o1) and floury1 (fl1) are normal in these regards. This suggests that other factors contribute to kernel texture. A liquid chromatography approach coupled with tandem mass spectrometry (LC-MS/MS) proteomics was used to compare non-zein proteins of nearly isogenic opaque endosperm mutants. In total, 2762 proteins were identified that were enriched for biological processes such as protein transport and folding, amino acid biosynthesis, and proteolysis. Principal component analysis and pathway enrichment suggested that the mutants partitioned into three groups: (i) Mc, DeB30, fl2 and o2; (ii) o1; and (iii) fl1. Indicator species analysis revealed mutant-specific proteins, and highlighted ER secretory pathway components that were enriched in selected groups of mutants. The most significantly changed proteins were related to stress or defense and zein partitioning into the soluble fraction for Mc, DeB30, o1, and fl1 specifically. In silico dissection of the most significantly changed proteins revealed novel qualitative changes in lysine abundance contributing to the overall lysine increase and the nutritional rebalancing of the o2 and fl2 endosperm.
- Subjects
ENDOSPERM; CORN; PLANT proteomics; LYSINE; ENDOPLASMIC reticulum
- Publication
Journal of Experimental Botany, 2016, Vol 67, Issue 5, p1381
- ISSN
0022-0957
- Publication type
Article
- DOI
10.1093/jxb/erv532