We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Potential of Lycii Radicis Cortex as an Ameliorative Agent for Skeletal Muscle Atrophy.
- Authors
Son, Rak Ho; Kim, Myeong Il; Kim, Hye Mi; Guo, Shuo; Lee, Do Hyun; Lim, Gyu Min; Kim, Seong-Min; Kim, Jae-Yong; Kim, Chul Young
- Abstract
Lycii Radicis Cortex (LRC) is a traditional medicine in East Asia with various beneficial effects, including antioxidant, anti-inflammatory, anti-tumor, anti-diabetic, and anti-depressant properties. However, its potential effects on skeletal muscle atrophy have not been studied. In this study, the protective effects of LRC extract (LRCE) on dexamethasone (DEX)-induced muscle atrophy were investigated in C2C12 myotubes and mice. We evaluated the effect of LRCE on improving muscle atrophy using a variety of methods, including immunofluorescence staining, quantitative polymerase chain reaction (qPCR), Western blot, measurements of oxidative stress, apoptosis, ATP levels, and muscle tissue analysis. The results showed that LRCE improved myotube diameter, fusion index, superoxide dismutase (SOD) activity, mitochondrial content, ATP levels, expression of myogenin and myosin heavy chain (MHC), and reduced reactive oxygen species (ROS) production in dexamethasone-induced C2C12 myotubes. LRCE also enhanced protein synthesis and reduced protein degradation in the myotubes. In mice treated with DEX, LRCE restored calf thickness, decreased mRNA levels of muscle-specific RING finger protein 1 (MuRF1) and atrogin-1, and increased insulin-like growth factor 1 (IGF-1) mRNA level. Moreover, LRCE also repaired gastrocnemius muscle atrophy caused by DEX. Although human studies are not available, various preclinical studies have identified potential protective effects of LRCE against muscle atrophy, suggesting that it could be utilized in the prevention and treatment of muscle atrophy.
- Subjects
EAST Asia; MUSCULAR atrophy; SOMATOMEDIN C; MUSCLE growth; PROTEOLYSIS; CALF muscles
- Publication
Pharmaceuticals (14248247), 2024, Vol 17, Issue 4, p462
- ISSN
1424-8247
- Publication type
Article
- DOI
10.3390/ph17040462