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- Title
Placental methylome analysis from a prospective autism study.
- Authors
Schroeder, Diane I.; Schmidt, Rebecca J.; Crary-Dooley, Florence K.; Walker, Cheryl K.; Ozonoff, Sally; Tancredi, Daniel J.; Hertz-Picciotto, Irva; M. LaSalle, Janine
- Abstract
Background: Autism spectrum disorders (ASD) are increasingly prevalent neurodevelopmental disorders that are behaviorally diagnosed in early childhood. Most ASD cases likely arise from a complex mixture of genetic and environmental factors, an interface where the epigenetic marks of DNA methylation may be useful as risk biomarkers. The placenta is a potentially useful surrogate tissue characterized by a methylation pattern of partially methylated domains (PMDs) and highly methylated domains (HMDs) reflective of methylation patterns observed in the early embryo. Methods: In this study, we investigated human term placentas from the MARBLES (Markers of Autism Risk in Babies: Learning Early Signs) prospective study by whole genome bisulfite sequencing. We also examined the utility of PMD/ HMDs in detecting methylation differences consistent with ASD diagnosis at age three. Results: We found that while human placental methylomes have highly reproducible PMD and HMD locations, there is a greater variation between individuals in methylation levels over PMDs than HMDs due to both sampling and individual variability. In a comparison of methylation differences in placental samples from 24 ASD and 23 typically developing (TD) children, a HMD containing a putative fetal brain enhancer near DLL1 was found to reach genome-wide significance and was validated for significantly higher methylation in ASD by pyrosequencing. Conclusions: These results suggest that the placenta could be an informative surrogate tissue for predictive ASD biomarkers in high-risk families.
- Subjects
AUTISM spectrum disorders; DISEASE prevalence; NEURODEVELOPMENTAL treatment; DIAGNOSIS
- Publication
Molecular Autism, 2016, Vol 7, p1
- ISSN
2040-2392
- Publication type
Article
- DOI
10.1186/s13229-016-0114-8