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- Title
Encapsulation of Apoptotic Proteins in Lipid Nanoparticles to Induce Death of Cancer Cells.
- Authors
Bae, Chun-Sik; Lee, Chang-Min; Ahn, Taeho
- Abstract
According to previous reports, cationic nanoparticles (cNPs) consisting of 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), a cationic lipid, without any cargos, induce generation of reactive oxygen species (ROS) and toxicity in cells. We investigated the effect of DOTAP-based cNPs containing encapsulated human pro-apoptotic proteins (P-cNPs), tBid, Bax, or cytochrome c (Cyt c) on the death of HepG2, cancer cells. Upon the treatment to cells, cNPs containing Cyt c-cargo were the most effective in inducing cell death, followed by tBid- and Bax-cNPs. Among three P-cNPs, Cyt c-cNPs also induced the highest levels of ROS production and caspase-3 activity in the cells under the treatment with the same DOTAP concentration. The incorporation of dioleoylglycerol (DOG), a neutral phospholipid, in cNPs at the expense of DOTAP resulted in an increased amount of the encapsulated protein and consequently enhanced cell death in a DOG concentration-dependent manner. Thus, the present cNP formulation with protein cargo may elicit apoptotic effects in cancer cells and serve as a rational background for the preparation of cationic lipid-based NP formulation for cancer treatment.
- Subjects
CANCER cells; CELL death; CATIONIC lipids; CYTOCHROME c; PROTEINS; REACTIVE oxygen species
- Publication
Biotechnology & Bioprocess Engineering, 2020, Vol 25, Issue 2, p264
- ISSN
1226-8372
- Publication type
Article
- DOI
10.1007/s12257-019-0409-7