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- Title
Multiplexed hydrogel microparticle suspension arrays for facile ribosomal RNA integrity assays.
- Authors
Duenas, Yader; Lee, JaeHun; Jung, Sukwon; Yi, Hyunmin
- Abstract
Reliable RNA integrity assay is important for a wide range of applications in genomics and diagnostics, yet the existing technologies have certain limitations such as large amount of sample required, high cost of equipment and/or long turnaround times. We report a simple assay method to analyze bacterial ribosomal RNA (rRNA) from complex total RNA samples utilizing shape-encoded and single-stranded DNA-conjugated hydrogel microparticle suspension arrays with no need for target amplification and under standard fluorescence imaging conditions. We show that our simple microparticle-based sensing scheme is reliable, sequence-specific and presents a responsive binding behavior to target RNA concentrations. Moreover, the relative stability of 16S and 23S rRNA can be assessed in a simple shape encoding-based multiplexed format. Combined, these findings represent a significant step toward cheap, fast, simple, and reliable assays for the analysis of rRNA and general RNA integrity.
- Subjects
RIBOSOMAL RNA; HYDROGELS; GENOMICS; MOLECULAR genetics; GENOMES; GENE amplification
- Publication
Biotechnology & Bioprocess Engineering, 2015, Vol 20, Issue 5, p956
- ISSN
1226-8372
- Publication type
Article
- DOI
10.1007/s12257-015-0265-z