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- Title
Recombinase-independent AAV for anterograde transsynaptic tracing.
- Authors
Faress, Islam; Khalil, Valentina; Yamamoto, Haruka; Sajgo, Szilard; Yonehara, Keisuke; Nabavi, Sadegh
- Abstract
Viral transsynaptic labeling has become indispensable for investigating the functional connectivity of neural circuits in the mammalian brain. Adeno-associated virus serotype 1 (AAV1) allows for anterograde transneuronal labeling and manipulation of postsynaptic neurons. However, it is limited to delivering an AAV1 expressing a recombinase which relies on using transgenic animals or genetic access to postsynaptic neurons. We reasoned that a strong expression level could overcome this limitation. To this end, we used a self-complementary AAV of serotype 1 (scAAV1) under a strong promoter (CAG). We demonstrated the anterograde transneuronal efficiency of scAAV1 by delivering a fluorescent marker in mouse retina-superior colliculus and thalamic-amygdala pathways in a recombinase-independent manner in the mouse brain. In addition to investigating neuronal connectivity, anterograde transsynaptic AAVs with a strong promoter may be suitable for functional mapping and imaging.
- Subjects
TRANSGENIC animals; NEURAL circuitry; ADENO-associated virus; FUNCTIONAL connectivity; SUPERIOR colliculus; RECOMBINASES
- Publication
Molecular Brain, 2023, Vol 16, Issue 1, p1
- ISSN
1756-6606
- Publication type
Article
- DOI
10.1186/s13041-023-01053-7