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- Title
A Major Latex-Like Protein Is a Key Factor in Crop Contamination by Persistent Organic Pollutants.
- Authors
Inui, Hideyuki; Sawada, Mami; Goto, Junya; Yamazaki, Kiyoshi; Kodama, Noriko; Tsuruta, Hiroki; Eun, Heesoo
- Abstract
This is the first report, to our knowledge, to reveal important factors by which members of the Cucurbitaceae family, such a cucumber (Cucumis sativus), watermelon (Citrullus lanatus), melon (Cucumis melo), pumpkin (Cucurbita pepo), squash (C. pepo), and zucchini (C. pepo), are selectively polluted with highly toxic hydrophobic contaminants, including organochlorine insecticides an dioxins. Xylem sap of C. pepo ssp. pepo, which is a high accumulator of hydrophobic compounds, solubilized the hydrophobi compound pyrene into the aqueous phase via some protein(s). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of xylem sap of two C. pepo subspecies revealed that the amount of 17-kD proteins in C. pepo ssp. pepo was larger than that in C. pepo ssp. ovifera, a low accumulator, suggesting that these proteins may be related to the translocation of hydrophobic compounds. The protein bands at 17 kD contained major latex-like proteins (MLPs), and the corresponding genes MLP-PG1, MLP-GR1, and MLP-GR cloned from the C. pepo cultivars Patty Green and Gold Rush. Expression of the MLP-GR3 gene in C. pepo cultivars was positiv correlated with the band intensity of 17-kD proteins and bioconcentration factors toward dioxins and dioxin-like compounds Recombinant MLP-GR3 bound polychlorinated biphenyls immobilized on magnetic beads, whereas recombinant MLP-PG1 a MLP-GR1 did not. These results indicate that the high expression of MLP-GR3 in C. pepo ssp. pepo plants and the existence of ML GR3 in their xylem sap are related to the efficient translocation of hydrophobic contaminants. These findings should be useful for decreasing the contamination of fruit of the Cucurbitaceae family as well as the phytoremediation of hydrophobic contaminants.
- Subjects
CUCURBITACEAE; ORGANOCHLORINE insecticides; XYLEM; GEL electrophoresis; PLANT proteins; PLANT genes; GENE expression in plants
- Publication
Plant Physiology, 2013, Vol 161, Issue 4, p2128
- ISSN
0032-0889
- Publication type
Article
- DOI
10.1104/pp.112.213645