Antibacterial, antidiarrheal, anti-inflammatory and analgesic activities of compound Shikuqin powder.

Bo Yu; Jiankang Yu; Lingling Jiang; Xiangxiu Chen; Xu Song; Sixuan Zhou; Jinge Xu; Jie Wu; Zhiqiang Tu; Zhiling Song; Zhongqiong Yin; Yishun Shang

The MIC and MBC values of Shikuqin (SKQ) against 5 bacteria that readily cause diarrhea were measured by the broth micro dilution method. The castor oil-induced diarrhea method was used to evaluate the antidiarrheal activity. Intestinal transit and gastric emptying were also evaluated with normal and neostigmine-induced intestinal transit in rodents. In addition, the antidiarrheal activity of SKQ was assessed in vivo with isolated rabbit ileum. Xylene-induced ear edema was used to evaluate the anti-inflammatory activities in mice, while hot plate and writhing tests were performed to assess the analgesic effects. Senna decoction (0.3g/mL) was administered intragastrically to induce a rat model of diarrhea. Semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to detect AQP4 mRNA, and Western blot was performed to quantify the protein level of AQP4 in the colon. SKQ exhibits remarkable antidiarrheal, anti-inflammatory and analgesic effects in the gastrointestinal tract disorders, and can therefore be developed as a promising antidiarrheal agent.

POWDERS; GASTRIC emptying; GASTROINTESTINAL system; WESTERN immunoblotting; DIARRHEA

Pakistan Journal of Pharmaceutical Sciences, 2019, Vol 32, p1333

1011-601X

Academic Journal