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- Title
Sodium-potassium ATPase activity mediates cyst formation in metanephric organ culture.
- Authors
Avner, Ellis D.; Sweeney Jr., William E.; Finegold, David N.; Piesco, Nicholas P.; Ellis, Demetrius
- Abstract
To study the possible role of altered transtubular transport in renal tubular cyst formation, the ontogeny of renal Na-K ATPase was studied during glucocorticoid-induced cystic metanephric tubular development in serum-free, murine organ culture (SFMOC). Utilizing an enzyme-linked kinetic microassay, a developmental profile of total ATPase and specific Na-K ATPase activity was established for control (CON) and glucocorticoid-induced cystic organ culture (CY) explants. During 120 hr of CON and CY organ culture nephrogenesis total Na-K ATPase activity, specific Na-K ATPase activity, and the Na-K ATPase:total ATPase ratio progressively increased, simulating normal in vivo murine enzyme development. However, from 48 to 120 hr of organ culture. CY showed significant increases in Na-K ATPase activity when compared to CON at similar stages of development, Na-K ATPase activity (expressed as nmoles · min-1 · mg protein-1, mean ± SD) was, at: 48 hr, CY 13.1 ± 0.7 vs. CON 11.0 ± 0.9 (P < 0.01);72 hr, CY 16.4 ± 1.1 vs. CON 12.2 ± 0.7 (P < 0.001): 96 hr. CY 35.4 ± 4.9 vs. CON 13.7 ± 0.4 (P < 0.001); and 120 hr. CY 26.1 ± 1.4 vs. CON 16.3 ± 0.9 (P < 0.001), The initial differences in CY enzyme activity preceded the earliest ultrastructural evidence of cyst formation by 18 to 24 hr. while subsequent increases in Na-K ATPase activity In CY paralleled progressive tubular cyst formation. Tubular cyst formation in CY could be largely prevented by daily incubation of explants with ouabain, 0.2 mM (final concentration) × 120 min, without deleterious effects on overall metanephric development. We conclude that increased Na-K ATPase activity has a primary role in tubular cyst formation in the SFMOC model.
- Subjects
ADENOSINE triphosphatase; RENAL tubular transport; CYSTIC kidney disease; GLUCOCORTICOIDS; KIDNEY diseases; PATHOLOGICAL physiology
- Publication
Kidney International, 1985, Vol 28, Issue 3, p447
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1038/ki.1985.151