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- Title
Reevaluation of the resting time period when preparing whole blood–derived platelet concentrates with the platelet-rich plasma method.
- Authors
Moroff, Gary; Kline, Linda; Dabay, Michelle; Hunter, Suzanne; Johnson, Adrienne; McNeil, Deanna; Nixon, Jan; Sawyer, Sherrie; Taylor, Harry; Whitley, Pam; Wahab, Fawzi; Murphy, Scott
- Abstract
BACKGROUND: The resting of platelet (PLT) pellets during the preparation of whole blood–derived PLT concentrates (PCs) is considered an essential step. A reevaluation of the rest period was conducted because preparation and storage conditions have been modified during the past 20 years. STUDY DESIGN AND METHODS: A two-site in vitro study (Study 1) was conducted with rest times of 0 to 5 minutes, 1 hour, and 4 hours (n = 31-33 per rest period). Leukoreduced PCs were stored for 5 days. A second study (Study 2) measuring in vivo viability was conducted at a third site (14 paired studies). PCs were prepared from 2 units of whole blood on the same day to simultaneously compare viability following storage and radioisotopic labeling. RESULTS: In Study 1, comparable in vitro parameters and swirling were observed with the three rest periods. The mean (±1 SD) values after storage for the extent of shape change and hypotonic stress parameters were 0 to 5 minutes, 16.7 ± 7.2 and 66.0 ± 15.7%; 1 hour, 19.1 ± 6.9 and 69.3 ± 17.9%; and 4 hours, 17.6 ± 5.5 and 64.1 ± 11.3%. In Study 2, the in vivo recovery was 49.9 ± 15.3 and 50.9 ± 20.2% with 0- to 5-minute and 1-hour rest periods, respectively. The corresponding survival time was 111.2 ± 50.7 and 114.9 ± 43.8 hours. CONCLUSION: These studies indicate comparable in vitro and in vivo viability properties with 0- to 5-minute and 1-hour rest periods and at 4 hours (in vitro only).
- Subjects
BLOOD collection; RADIOLABELED blood platelets; BLOOD testing; RADIOACTIVE tracers; MICROBIAL viability counts
- Publication
Transfusion, 2006, Vol 46, Issue 4, p572
- ISSN
0041-1132
- Publication type
Article
- DOI
10.1111/j.1537-2995.2006.00756.x