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- Title
Gene Mapping and Identification of a Missense Mutation in One Copy of VRN-A1 Affects Heading Date Variation in Wheat.
- Authors
Xue, Qianwen; Xiong, Hongchun; Zhou, Chunyun; Guo, Huijun; Zhao, Linshu; Xie, Yongdun; Gu, Jiayu; Zhao, Shirong; Ding, Yuping; Xu, Le; Liu, Luxiang
- Abstract
Heading date (HD) is an important trait for wide adaptability and yield stability in wheat. The Vernalization 1 (VRN1) gene is a key regulatory factor controlling HD in wheat. The identification of allelic variations in VRN1 is crucial for wheat improvement as climate change becomes more of a threat to agriculture. In this study, we identified an EMS-induced late-heading wheat mutant je0155 and crossed it with wide-type (WT) Jing411 to construct an F2 population of 344 individuals. Through Bulk Segregant Analysis (BSA) of early and late-heading plants, we identified a Quantitative Trait Locus (QTL) for HD on chromosome 5A. Further genetic linkage analysis limited the QTL to a physical region of 0.8 Mb. Cloning and sequencing revealed three copies of VRN-A1 in the WT and mutant lines; one copy contained a missense mutation of C changed to T in exon 4 and another copy contained a mutation in intron 5. Genotype and phenotype analysis of the segregation population validated that the mutations in VRN-A1 contributed to the late HD phenotype in the mutant. Expression analysis of C- or T-type alleles in exon 4 of the WT and mutant lines indicated that this mutation led to lower expression of VRN-A1, which resulted in the late-heading of je0155. This study provides valuable information for the genetic regulation of HD and many important resources for HD refinement in wheat breeding programs.
- Subjects
GENE mapping; MISSENSE mutation; WHEAT genetics; LOCUS (Genetics); WHEAT breeding; GENETIC regulation; WHEAT; MOLECULAR cloning
- Publication
International Journal of Molecular Sciences, 2023, Vol 24, Issue 5, p5008
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms24055008