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- Title
Effects of autoantibodies against the angiotensin II receptor on rat ventricular myocardial ionic currents.
- Authors
Xiao-Li Yang; Peng Wang; Jie Zhang; Li Yan; Ye Wu; Xin-Liang Ma; Hui-Rong Liu
- Abstract
Objectives: The importance of agonistic antibodies against the angiotensin II receptor (AT1-AA) in the pathogenesis of preeclampsia has not been well researched. The aims of our study ware to 1) obtain direct evidence that the concentration of AT1-AA is increased in preeclampsia; 2) determine the effects of AT1-AA on ventricular ionic currents. Methds: Sera obtained from preeclamptic women (n = 18) were applied to detect AT1-AA and purify IgG and those collected from normal pregnant women (n = 17) were used as negative control. The effects of AT1-AA on rat ventricular ionic currents were studied by whole cell patch clamp techniques. Results: 1) the concentration of AT1-AA is time-dependently increased in preeclampsia compared with normal pregnant women. 2) AT1-AA could dose-dependently increase both IC a -L and IN a/C a current which blocked by losartan, a specific antagonist of AT1-receptor suggesting that the agonist-like activity of AT1-AA are mediated by stimulation of AT1-receptor. 3) AT1-AA decreased dose-dependently both Ito and 1 k1current which were also blocked by losartan, However these effects were not observed when IgG from normal pregnant women were administered. Conclusion: Our study provides direct evidence that the concentration of AT1-AA is markedly increased in preeclampsia; it has a remarkable positive inotropic effect on the heart, indicating that it may be play an important role in the pathogenesis of preeclampsia.
- Subjects
AUTOANTIBODIES; ANGIOTENSIN II; PREECLAMPSIA; ION flow dynamics; VENTRICULAR remodeling; PREGNANCY
- Publication
FASEB Journal, 2007, Vol 21, Issue 5, pA539
- ISSN
0892-6638
- Publication type
Article