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- Title
Disordered Regions of Mixed Lineage Leukemia 4 (MLL4) Protein Are Capable of RNA Binding.
- Authors
Szabó, Beáta; Murvai, Nikoletta; Abukhairan, Rawan; Schád, Éva; Kardos, József; Szeder, Bálint; Buday, László; Tantos, Ágnes
- Abstract
Long non-coding RNAs (lncRNAs) are emerging as important regulators of cellular processes and are extensively involved in the development of different cancers; including leukemias. As one of the accepted methods of lncRNA function is affecting chromatin structure; lncRNA binding has been shown for different chromatin modifiers. Histone lysine methyltransferases (HKMTs) are also subject of lncRNA regulation as demonstrated for example in the case of Polycomb Repressive Complex 2 (PRC2). Mixed Lineage Leukemia (MLL) proteins that catalyze the methylation of H3K4 have been implicated in several different cancers; yet many details of their regulation and targeting remain elusive. In this work we explored the RNA binding capability of two; so far uncharacterized regions of MLL4; with the aim of shedding light to the existence of possible regulatory lncRNA interactions of the protein. We demonstrated that both regions; one that contains a predicted RNA binding sequence and one that does not; are capable of binding to different RNA constructs in vitro. To our knowledge, these findings are the first to indicate that an MLL protein itself is capable of lncRNA binding.
- Subjects
RIBONUCLEASES; ENDORIBONUCLEASES; EXORIBONUCLEASES; NUCLEASES; SARCIN
- Publication
International Journal of Molecular Sciences, 2018, Vol 19, Issue 11, p3478
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms19113478