We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Epac- and Rap-independent ERK1/2 phosphorylation induced by G<sub>s</sub>-coupled receptor stimulation in HEK293 cells
- Authors
Norum, Jens Henrik; Dawood, Hoda; Mattingly, Raymond R.; Sandnes, Dagny; Levy, Finn Olav
- Abstract
Abstract: Serotonin activates Ras and Ras-dependent ERK1/2 phosphorylation in HEK293 cells expressing Gs-coupled 5-HT4 or 5-HT7 serotonin receptors through unknown mechanisms. Both Epac/Rap-dependent and -independent pathways for Ras-dependent ERK1/2 activation have been suggested. Epac overexpression or Epac-specific 8-CPT-2′-O-Me-cAMP did not cause ERK1/2 phosphorylation, despite Rap activation. The data did not support a role for PLCε or DAG-dependent Ras GEFs of the Ras-GRP family in Ras-dependent ERK1/2 phosphorylation. However, serotonin stimulated phosphorylation of endogenous and recombinant Ras-GRF1, increased [Ca2+]i and caused Ca2+- and calmodulin-dependent ERK1/2 phosphorylation. Different signalling pathways seem to be utilised by Gs-coupled receptors in various isolates of HEK293 cells.
- Subjects
SEROTONIN; PHOSPHORYLATION; CELLS; CALMODULIN
- Publication
FEBS Letters, 2007, Vol 581, Issue 1, p15
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2006.11.069