We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Level of M(IP)<sub>2</sub>C sphingolipid affects plant defensin sensitivity, oxidative stress resistance and chronological life-span in yeast
- Authors
Aerts, An M.; François, Isabelle E.J.A.; Bammens, Leen; Cammue, Bruno P.A.; Smets, Bart; Winderickx, Joris; Accardo, Sabina; De Vos, Dirk E.; Thevissen, Karin
- Abstract
Abstract: The antifungal plant defensin DmAMP1 interacts with fungal sphingolipids of mannosyldiinositolphosphorylceramide (M(IP)2C) class. We screened a Saccharomyces cerevisiae transposon (Tn) mutant library against DmAMP1 and identified one DmAMP1-resistant mutant with the Tn inserted in the M(IP)2C biosynthesis gene IPT1 (DmTn11) and one DmAMP1-hypersensitive mutant with the Tn inserted in rDNA (HsTnII). However, tetrad analysis pointed to HsTnII as a spontaneous mutant. Apparently, membranes of DmTn11 lack M(IP)2C, whereas membranes of HsTnII have increased M(IP)2C levels. In addition, DmTn11 and HsTnII are characterized by increased and reduced oxidative stress resistance/chronological life-span (CL), respectively. A putative involvement of M(IP)2C in oxidative stress and CL in yeast is discussed.
- Subjects
SPHINGOLIPIDS; SACCHAROMYCES cerevisiae; OXIDATIVE stress; YEAST
- Publication
FEBS Letters, 2006, Vol 580, Issue 7, p1903
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2006.02.061