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- Title
Bovine PrP<sup>C</sup> directly interacts with αB-crystalline
- Authors
Sun, Guihong; Guo, Mingxiong; Shen, Ao; Mei, Fanghua; Peng, Xiaoxue; Gong, Rui; Guo, Deyin; Wu, Jianguo; Tien, Po; Xiao, Gengfu
- Abstract
Abstract: We used a bovine brain cDNA library to perform a yeast two-hybrid assay with bovine mature PrPC as bait. The screening result showed that αB-crystalline interacted with PrPC. The interaction was further evaluated both in vivo and in vitro with different methods, such as immunofluorescent colocalization, native polyacrylamide-gel electrophoresis, and IAsys biosensor assays. The results suggested that αB-crystalline may have the ability to refold denatured prion proteins, and provided first evidence that αB-crystalline is directly associated with prion protein.
- Subjects
CREUTZFELDT-Jakob disease; BOVINE spongiform encephalopathy; VIRUS diseases in cattle; ELECTROPHORESIS
- Publication
FEBS Letters, 2005, Vol 579, Issue 24, p5419
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2005.08.065