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- Title
NIRF is a ubiquitin ligase that is capable of ubiquitinating PCNP, a PEST-containing nuclear protein
- Authors
Mori, Tsutomu; Li, Yuanyuan; Hata, Hiroaki; Kochi, Hideo
- Abstract
We previously reported the association of a novel Np95/ICBP90-like RING finger protein (NIRF) with a novel PEST-containing nuclear protein (PCNP). NIRF is a nuclear protein with a ubiquitin-like domain, a PHD finger, a YDG/SRA domain, Rb-binding motifs and a RING finger. In this study, we showed that NIRF has auto-ubiquitination activity, the hallmark of a ubiquitin ligase. PCNP was readily ubiquitinated in 293 and COS-7 cells, and NIRF ubiquitinated PCNP in vitro as well as in vivo. Considering that NIRF is implicated in cell cycle regulation, these findings suggest that NIRF and PCNP are a ubiquitin ligase and its substrate, respectively, and may constitute a novel signaling pathway with some relation to cell proliferation.
- Subjects
PROTEINS; UBIQUITIN; CELL proliferation; CELL cycle
- Publication
FEBS Letters, 2004, Vol 557, Issue 1-3, p209
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(03)01495-9