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- Title
Ca<sup>2+</sup>-induced oxidative stress in brain mitochondria treated with the respiratory chain inhibitor rotenone
- Authors
Sousa, Solange C.; Maciel, Evelise N.; Vercesi, Anibal E.; Castilho, Roger F.
- Abstract
In this study we show that micromolar Ca2+ concentrations (>10 μM) strongly stimulate the release of reactive oxygen species (ROS) in rotenone-treated isolated rat forebrain mitochondria. Ca2+-stimulated mitochondrial ROS release was associated with membrane lipid peroxidation and was directly correlated with the degree of complex I inhibition by rotenone. On the other hand, Ca2+ did not increase mitochondrial ROS release in the presence of the complex I inhibitor 1-methyl-4-phenylpyridinium. Cyclosporin A had no effect on Ca2+-stimulated mitochondrial ROS release in the presence of rotenone, indicating that mitochondrial permeability transition is not involved in this process. We hypothesized that Ca2+-induced mitochondrial oxidative stress associated with partial inhibition of complex I may be an important factor in neuronal cell death observed in the neurodegenerative disorder Parkinson’s disease.
- Subjects
MITOCHONDRIA; CALCIUM
- Publication
FEBS Letters, 2003, Vol 543, Issue 1-3, p179
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(03)00421-6