We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Improvement of Fucosylated Oligosaccharides Synthesis by α-L-Fucosidase from Thermotoga maritima in Water-Organic Cosolvent Reaction System.
- Authors
Robles-Arias, Mónica A.; García-Garibay, Mariano; Alatorre-Santamaría, Sergio; Tello-Solís, Salvador R.; Guzmán-Rodriguez, Francisco; Gómez-Ruiz, Lorena; Rodríguez-Serrano, Gabriela; Cruz-Guerrero, Alma E.
- Abstract
The effects of water activity (aw), pH, and temperature on transglycosylation activity of α-L-fucosidase from Thermotoga maritima in the synthesis of fucosylated oligosaccharides were evaluated using different water-organic cosolvent reaction systems. The optimum conditions of transglycosylation reaction were the pH range between 7 and 10 and temperature 90–95 °C. The addition of organic cosolvent decreased α-L-fucosidase transglycosylation activity in the following order: acetone > dimethyl sulfoxide (DMSO) > acetonitrile (0.51 > 0.42 > 0.18 mM/h). However, the presence of DMSO and acetone enhanced enzyme-catalyzed transglycosylation over hydrolysis as demonstrated by the obtained transglycosylation/hydrolysis rate (rT/H) values of 1.21 and 1.43, respectively. The lowest rT/H was calculated for acetonitrile (0.59), though all cosolvents tested improved the transglycosylation rate in comparison to a control assay (0.39). Overall, the study allowed the production of fucosylated oligosaccharides in water-organic cosolvent reaction media using α-L-fucosidase from T. maritima as biocatalyst.
- Subjects
THERMOTOGA maritima; ACETONITRILE; ORGANIC solvents; HYDROLYSIS; ENZYMES; OLIGOSACCHARIDES; ACETONE; DIMETHYL sulfoxide
- Publication
Applied Biochemistry & Biotechnology, 2021, Vol 193, Issue 11, p3553
- ISSN
0273-2289
- Publication type
Article
- DOI
10.1007/s12010-021-03628-3