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- Title
Control of Grifola frondosa Morphology by Agitation and Aeration for Improving Mycelia and Exo-Polymer Production.
- Authors
Cui, Feng-Jie; Chen, Xiao-Xiao; Liu, Wei-Min; Sun, Wen-Jing; Huo, Shuhao; Yang, Yan
- Abstract
The present study describes the improved mycelia and exo-polymer production under control of Grifola frondosa morphology by changing the aeration rate and agitation intensity in a 25-L stirred fermentor. The aeration rate of 1.0 vvm yielded a highest mycelia biomass of 24.754 g/L with the lowest pellet percentage of 20.5 %. The maximum exo-polymer (2.324 g/L) was achieved at 0.75 vvm with mycelia polysaccharide production (0.321 g/g), whereas clumps and filaments dominated the ratios of 45.6 and 33.9 %, respectively. The change of aeration rate and agitation intensity had slight influence on the monosaccharide compositions in exo-polymers and significantly affected glucose and mannose contents in the mycelia polysaccharides. These findings will provide a clue for exploring the relationship between fermentation parameters, morphologies, and polysaccharide synthesis pathway of G. frondosa.
- Subjects
MAITAKE; WATER aeration; POLYSACCHARIDES; AGONOMYCETALES; MANNOSE
- Publication
Applied Biochemistry & Biotechnology, 2016, Vol 179, Issue 3, p459
- ISSN
0273-2289
- Publication type
Article
- DOI
10.1007/s12010-016-2006-y