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- Title
A Rapid DNA Minipreparation Method Suitable for AFLP and Other PCR Applications.
- Authors
Chen, D.-H; Ronald, P.C.
- Abstract
Abstract. A rapid DNA minipreparation method was developed for rice and other plant species. This method uses an Eppendorf tube and 1-mi pipette tip to grind plant tissues, and requires only one transfer for DNA isolation. In a single day, one person can complete DNA isolation from more than 120 leaf samples. The yields of the DNA samples ranged from 2.3 to 5.2 Mu g from 25-50 mg fresh leaf tissue. DNA samples extracted using this method from rice were completely digested with five restriction enzymes (EcoR I, EcoR V, Hind III, Mse I and Pst I) and were successfully used for AFLP and other PCR applications.
- Subjects
DNA; RICE; PLANTS
- Publication
Plant Molecular Biology Reporter, 1999, Vol 17, Issue 1, p53
- ISSN
0735-9640
- Publication type
Article
- DOI
10.1023/A:1007585532036