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- Title
Creation of a Yeast Strain with Co‐Translationally Acylated Nucleosomes.
- Authors
Wu, Dan; Zhang, Yunfeng; Tang, Zhiheng; Chen, Xiaoxu; Ling, Xinyu; Li, Longtu; Cao, Wenbing; Zheng, Wei; Wu, Jiale; Tang, Hongting; Liu, Xiaoyun; Luo, Xiaozhou; Liu, Tao
- Abstract
Structurally diverse acylations have been identified as post‐translational modifications (PTMs) on histone lysine residues, but their functions and regulations remain largely unknown. Interestingly, in nature, a lysine acylation analog, pyrrolysine, is introduced as a co‐translational modification (CTM) through genetic encoding. To explore this alternative life form, we created a model organism Saccharomyces cerevisiae containing site‐specific lysine CTMs (acetyl‐lysine, crotonyl‐lysine, or another synthetic analog) at histone H3K56 using non‐canonical amino acid mutagenesis to afford a chemically modified nucleosome in lieu of their own in vivo. We further demonstrated that acetylation of histone H3K56 partly tends to provide a more favorable chromatin environment for DNA repair in yeast compared to crotonylation and crosstalk with other PTMs differently. This study provides a potentially universal approach to decipher the consequences of different histone lysine PTMs in eukaryotes.
- Subjects
POST-translational modification; CHROMATIN; YEAST; DNA repair; HISTONE acetylation; HISTONES
- Publication
Angewandte Chemie, 2022, Vol 134, Issue 30, p1
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/ange.202205570