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- Title
Quantitation of differential renal blood flow and renal function using dynamic contrast-enhanced MRI in rats.
- Authors
Pedersen, Michael; Shi, Yimin; Anderson, Peter; Stødkilde-Jørgensen, Hans; Christian Djurhuus, Jens; Gordon, Isky; Frøkiær, Jørgen
- Abstract
A first-pass upslope approach was used to estimate differential renal blood flow (DRBF) and a Patlak-Rutland approach was used to estimate differential renal function (DRF) using Gd-DTPA-BMA-enhanced MRI. DRBF and DRF were estimated in rat kidneys under three different experimental conditions: 1) transient renal artery occlusion (TRAO); 2) partial unilateral ureteric obstruction (PUO); and 3) sham-operated control rats (SHAM). A bolus of Gd-DTPA-BMA was given intravenously during a dynamic single slice T1-weighted gradient echo sequence, which allowed calculation of concentration from signal intensity values. Calculations based on the raw signal intensity showed that DRBF was decreased in both PUO (44 ± 1%; P < 0.05) and in TRAO (38 ± 1%; P < 0.05) compared with SHAM (52 ± 1%). Converting the signal intensity into a measure of Gd-DTPA-BMA concentration did not substantially alter these findings (PUO: 40 ± 3%, P < 0.05; TRAO: 35 ± 2%, P < 0.05; SHAM 49 ± 1%). Likewise, DRF decreased in both PUO (43 ± 4%; P < 0.05) and TRAO (39 ± 3%; P < 0.05) compared with SHAM (48 ± 2%). Converting the signal intensity into measurements of Gd-DTPA-BMA concentration revealed similar findings (PUO: 41 ± 5%, P < 0.05; TRAO: 34 ± 5%, P < 0.05; SHAM: 49 ± 2%). Our results suggest that renal damage in rats may be demonstrated by an observed reduction of DRBF and DRF as estimated from single-slice Gd-DTPA-BMA enhanced signal intensity using time-activity curves with and without quantitation of Gd-concentration. Magn Reson Med 51:510-517, 2004. © 2004 Wiley-Liss, Inc.
- Publication
Magnetic Resonance in Medicine, 2004, Vol 51, Issue 3, p510
- ISSN
0740-3194
- Publication type
Article
- DOI
10.1002/mrm.10711