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- Title
Expansion of transgenic tobacco protoplasts expressing pumpkin ascorbate oxidase is more rapid than that of wild-type protoplasts.
- Authors
Kato, Naohiro; Esaka, Muneharu
- Abstract
When pumpkin (Cucurbita spp., cv. Ebisu Nankin) ascorbate oxidase cDNA was introduced into cultured cells of tobacco BY-2 (Nicotiana tabacum L. cv. Bright Yellow No. 2) by Agrobacterium-mediated transformation, the transgenic cells expressed and secreted the recombinant pumpkin ascorbate oxidase into the culture medium. These transgenic cells showed no morphological difference from wild-type cells. However, in the presence of applied hormones protoplasts prepared from the transgenic cells elongated more rapidly than those of wild-type cells. We propose that ascorbate oxidase may play a key role in the regulation of cell expansion perhaps by controlling transport processes through the plasma membrane, but not by affecting the cell wall.
- Subjects
CUCURBITA pepo; PLANT plasma membranes; AGROBACTERIUM; ENDOCRINE glands; PROTOPLASTS; PUMPKINS
- Publication
Planta: An International Journal of Plant Biology, 2000, Vol 210, Issue 6, p1018
- ISSN
0032-0935
- Publication type
Article
- DOI
10.1007/s004250050712